Figure 7

Emission fluorescence scans at λ _ex 295 nm of VP6 _NT and VP6 _U (0.4 mg/mL) after MCO with 150 µM of FeCl ₂ at various H ₂ O ₂ concentrations for 1 h. A) Normalized emission spectra of VP6_NT. B) Normalized emission spectra of VP6_U. C) The center of spectral mass (CSM) of oxidized VP6_NT and VP6_U was calculated using Equation (3) and plotted against the H₂O₂ concentration used. On the right, the CSM of denatured VP6 (treated for 2 hours with 6 M of guanidine chloride) is shown for comparison. Experiments were performed in triplicate, except for the CSM of denatured VP6, which was analyzed in duplicate. Error bars represent the standard deviation or difference among them.