Figure 1

Characterization of purified VP6 _NT . A) 12% SDS-PAGE gel stained with Coomassie Blue (under reducing conditions): Lane 1, Molecular weight marker Benchmark (Life Technologies Corp., Carlsbad, CA, USA), the more intense band corresponds to 50 KDa. Lane 2, VP6_NT. B) Size exclusion chromatography, Peak 1 corresponds to VP6_NT, peak 2 to VP6_U and peak 3 to salts. K_D refers to the relative elution volume calculated with Equation 1. C) Transmission electronic micrograph at 85,000X. D) Intrinsic fluorescence spectrum of 40 µg/mL of VP6_NT or VP6_U. Excitation at 280 nm.