Figure 2

Change of the intracellular proteome of P. pastoris X-33 in response to secretory insulin precursor production with special attention to ERAD and UPR related proteins. (A) Sections of 2D gels containing most of ERAD and UPR related proteins are shown: samples taken at 0, 6, 18 and 48 h after the onset of the methanol fed-batch phase. (B) Abundance changes of ERAD and UPR related proteins are given in relative units corresponding to an average of the resulting values from duplicate gels. The protein (spot) related to the fragment of SSA3 ~25 kDa is shown in the small box. The small map indicates the position of the 2D sections in the entire 2D gel. Two representative 2D gels from cell samples taken at the end of the glycerol batch phase and during methanol-induced secretory IP production indicating the position of all identified proteins are given in the Additional file 1: Figure S1. Time course 2D data from a replicate cultivation are given in the Additional file 1: Figure S2.