Figure 3
From: Generation of human ER chaperone BiP in yeast Saccharomyces cerevisiae
Purification of secreted recombinant human BiP protein from yeast culture medium. M – unstained protein ladder (ThermoScientific, cat. no. 26614). A – crude yeast growth medium (20× concentrated), B – yeast growth medium after microfiltration (20× concentrated), C – 20× concentrated proteins from yeast growth medium in binding buffer after tangential ultrafiltration; D – purified yeast-derived recombinant human BiP protein (5 μg).