Table 5 Primers used in this study

LP1

5’-ATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACAGCGATTGTGTAGGCTGGAG-3’, deletion of aroL

LP2

5’-TCAACAATTGATCGTCTGTGCCAGGGCGCTGCGAATTTCAGAAATCACCTTAACGGCTGACATGGGAATTAG-3’, deletion of aroL

P1

5’-GTTTCGTTGGCATCGTTCTT-3’, Diagnostic PCR for the deletion of aroL

P2

5’-ATTCTCATGACACCGGCTTT-3’, Diagnostic PCR for the deletion of aroL

KP1

5’-ATGGCAGAGAAACGCAATATCTTTCTGGTTGGGCCTATGGGTGCCGGAAAAGCGATTGTGTAGGCTGGAG-3’, deletion of aroK

KP2

5’-TTAGTTGCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTAACGGCTGACATGGGAATTAG-3’, deletion of aroL

P3

5’-GCGAAGCGGGTTTATCATTA-3’, Diagnostic PCR for the deletion of aroK

P4

5’-GTTCCCCGAGAGTAACGAC-3’, Diagnostic PCR for the deletion of aroK

GP1

5’-GCCTGCAGAGGAGGGCGTAAATATGAATTATCAGAACGACGATT-3’, Pst I, PCR for aroG

GP2

5’-CGGCATGCTTACCCGCGACGCGCTTTTACT-3’, Sph I, PCR for aroG

G15P1

5’-CTCAATATGATCACCCCACAAT-3’, sited-specific mutagenesis of aroG

G15P2

5’-AAACTCACCTGCCGCTGGCAGACCG-3’, sited-specific mutagenesis of aroG

EP1

5’-GCGAATTCAGGAGG TAATAAATATGGAAACCTAT GCTGTTTT-3’, Eco RI, PCR for aroE

EP2

5’-CGGCGGCCGCTTAT CACGCGGACAATTCCTCCT-3’, Not I, PCR for aroE

BP1

5’-CGGCGGCCGCAGGAGG TAATAAATATGGAGAGGATTGTCGTTAC-3’, Not I, PCR for aroB

BP2

5’-GCCCATGGTTACGCTGATTGACAATCGG-3’, Nco I, PCR for aroB

TP1

5’-GCGCATGCAGGAGG TAATAAATATGTCCTCACG TAAAGAGCT-3’, Sph I, PCR for tktA

TP2

5’-CGGAGCTCTTACAGCAGTTCTTTTGCTTTCG-3’, Sac I, PCR for tktA

PntF

5’-CAGGGTACCTCATCAATAAAACCG-3’, Kpn I, PCR for pntAB

PntR

5’-CGTCTGCAGTTACAGAGCTTTCAG-3’, Pst I, PCR for pntAB

SthF

5’-TTTTGGTACCCAGGTAAGCCCTACCATGC-3’, Kpn I, PCR for sthA

SthR

5’-GGGCTGCAGGGCCATTTCGATAAAGTTTT-3’, Pst I, PCR for sthA

NadF

5’-GCGGGGTACCATGAATAATCATTTCAAGTG-3’, Kpn I, PCR for nadK

NAdR

5’-GCGGTCTAGATTAGAATAATTTTTTTGACCA-3’, Xba I, PCR for nadK

5TacF

5’-ACGCGTGTAAAACGACGGCCAGT-3’, Mlu I, PCR for the 5P_tac promoter

5TacR

5'-CCGCGCATGCGGATCCGAATTCATGCATCTAGTATTTCTCCTCTTTAATGGAT-3’, Sph I, PCR for the 5P_tac promoter

CsrF

5’-CGGAGCTCAGGAGGTAATAAATGAGTCAGACAACGAAGTGAACAT-3’, Sac I, PCR for the csrB and pps gene cluster

PpsR

5’-CGGGTACCTTATTTCTTCAGTTCAGCCAGG-3’, Kpn I, PCR for the csrB and pps gene cluster

QEF

5’-GGATCGCCGGAATATCACCAC-3’, qPCR for the aroE gene

QER

5’-ACTACTGCCACTCCTTTCCCT-3’, qPCR for the aroE gene

QBF

5’-AAC GAA ACC CTG GCT CCT CTG-3’, qPCR for the aroB gene

QBR

5’-AAGCGCCACCAGCGTAGTATC-3’, qPCR for the aroB gene

QGF

5’-CGTTGCTGAAGTGAAAGAAGGG-3’, qPCR for the aroG gene

QGR

5’-ACGTCAGCACAAACATCCATC-3’, qPCR for the aroG gene

QTF

5’-TTTCGCCTGGCCTGCTTCTTT-3’, qPCR for the tktA gene

QTR

5’-CGACGCTGAAATTGCCCTGAC-3’, qPCR for the tktA gene

QNF

5’-TGGAATCAACCGTGGCAACCT-3’, qPCR for the nakK gene

QNR

5’-TGGAATCAACCGTGGCAACCT-3’, qPCR for the nakK gene

QPF

5’-AGCCGGAGTACGAGTTCAGCA-3’, qPCR for the pntAB gene

QPR

5’-ATTGCGCTGGTATTCGGCTGG-3’, qPCR for the pntAB gene

QPPF

5’-GACATCTTCTCGCTGACCAAC-3’, qPCR for the ppsA g ene

QPPR

5’-TTACCGGTGTGGCCATCTTTC-3’, qPCR for the ppsA gene

QCSF

5’-CTGGATGAAGCGAAGAGGATG-3’ , qPCR for the csrB gene

QCSR

5’-ATTGCTTCCTGCTCACACCAC-3’, qPCR for the csrB gene

QCF

5’-TTGTCGGCGGTGGTGATGTC-3’, qPCR for the cysG gene

QCR

5’-ATGCGGTGAACTGTGGAATAAACG-3’, qPCR for the cysG gene