Table 4 Strains and plasmids used in this study

Strain

E. coli DH5α

supE44 Δ(lacZYA-argF) U169 (Φ80lacZ ΔM15) hsdR17 recA endA1 gyrA96 thi-1 relA1

Invitrogen

E. coli BW25113

lacI ^q rrnB _T14 ΔlacZ _WJ16 hsdR514 ΔaraBAD _AH33 ΔrhaBAD _LD78

28

E. coli BW25113 (ΔaroK ΔaroL)

E. coli BW25113, ΔaroK ΔaroL

This study

E. coli BW25113 (ΔaroK ΔaroL, P _pps ::P _lacQ1 , P _csrB ::P _lacQ1 )

E. coli BW25113, ΔaroK ΔaroL, replacement of the native promoter of the pps and csrB gene with the P _lacQ1 promoter

This study

E. coli BW25113 (ΔaroK ΔaroL, P _pps ::P _lacQ1 , P _csrB ::P _lacQ1 , P _T5 -pps, P _T5 -csrB)

E. coli BW25113(ΔaroK ΔaroL, P _pps ::P _lacQ1 , P _csrB ::P _lacQ1 ) with an additional chromosomal copy of the pps and csrB genes under the control of the T5 promoter

This study

SA110

CIChE strain of the aroG ^fbr , tktA, aroB and aroE gene cluster resistance to 2 μM triclosan from E. coli BW25113 (ΔaroK ΔaroL, P _pps ::P _lacQ1 , P _csrB ::P _lacQ1 ), ΔrecA

This study

SA112

SA110 with an additional chromosomal copy of the tktA gene under the control of the 5P_tac promoter (5 tandem repeats of the core-tac-promoter)

This study

SA114

SA112 with an additional chromosomal copy of the pntAB genes under the control of the 5P_tac promoter

This study

SA116

SA112 with an additional chromosomal copy of the nadK gene under the control of the 5P_tac promoter

This study

Plasmid

pHKKF3T5b

CIChE integration expression vector, attP _HK site, Kan^r

16

pHKKT5b

Integration expression plasmid, attP _HK site, P_T5 promoter, Kan^r

33,34

pP21KT5b

Integration expression plasmid, attP _P21 site, P_T5 promoter, Kan^r

33,34

pAH69

Helper plasmid expressing phage HK022 Int, Amp^r

31

pAH121

Helper plasmid expressing phage P21 Int, Amp^r

31

pCP20

pSC101 replicon^ts Flp(λRp) cI 857, Cm^r, Amp^r

28

pKD3

oriRγ, FRT::cat::FRT template plasmid, Cm^r, Amp^r

28

pSIM6

pSC101 replicon^ts P_L -gam-bet-exo cI 857, Amp^r

29

p5TG

pSC101 replicon^ts, 5P_tac promoter, Spc^r

32

pMP5

P5TG derivative, pSC101 ori, Constitutive expression, Spc^r

This study

pBAD24

pMB1 ori, P_BAD L-arabinose inducible, Amp^r

30

pBAD-csrB-pps

pBAD24 derivative containing the csrB and pps genes

Lab storage

pBEB

pBAD24 derivative containing the aroB and aroE genes

This study

pBEBG

pBAD24 derivative containing the aroG ^fbr , aroB and aroE genes

This study

pHKEBG

pHKKF3T5b derivative containing aroG ^fbr , aroB and aroE genes

This study

pHKEBGT

pHKKF3T5b derivative containing aroG ^fbr , tktA, aroB and aroE genes

This study

pMPsthA

pMP5 derivative containing sthA gene

This study

pMPpntAB

pMP5 derivative containing pntAB genes

This study

pMPnadK

pMP5 derivative containing nadK gene

This study

pP21KT5b-csrB-pps

pP21KT5b derivative containing csrB and pps genes

This study

pHKKT5b-tktA

pHKKT5b derivative containing tktA gene

This study

pHKK5Tacb

Integration expression plasmid, attP _HK site, 5tac promoter, Kan^r

This study

pP21K5Tacb

Integration expression plasmid, attP _P21 site, 5tac promoter, Kan^r

This study

pP21K5Tacb-pntAB

pHK5Tacb derivative containing pntAB genes

This study

pP21K5Tacb-nadk

pHK5Tacb derivative containing nadK gene

This study