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Table 2 Effect of over-expressing genes involved in NADPH synthesis on SA production in E. coli SA112

From: Production of shikimic acid from Escherichia coli through chemically inducible chromosomal evolution and cofactor metabolic engineering

Strain

OD600

SA concentration (g/L)

Yield (mol SA/mol glucose)

NADPH (μM)

SA112 (pMP5)

4.79 ± 0.64

1.34 ± 0.09

0.22

1.93 ± 0.01

SA112 (pMPsthA)

5.78 ± 0.26

1.42 ± 0.07

0.24

1.61 ± 0.01

SA112 (pMPpntAB)

5.50 ± 0.11

1.89 ± 0.01

0.27

2.23 ± 0.01

SA112 (pMPnadK)

5.20 ± 0.34

1.92 ± 0.01

0.29

2.55 ± 0.01

SA114

6.55 ± 0.30

2.99 ± 0.01

0.31

4.51 ± 0.01

SA116

6.40 ± 0.51

3.12 ± 0.01

0.33

5.77 ± 0.01