Skip to main content

Table 1 Plasmids and strains used in this study

From: Microbial production of sabinene—a new terpene-based precursor of advanced biofuel

Name Relevant characteristics References
pACYCDuet-1 P15A origin; CmR; PT7 Novagen
pTrcHis2B ColE1 origin; AmpR; Ptrc Invitrogen
pGH pUC origin; AmpR; PT7 Generay
pTrcLower ColE1 origin; AmpR; Ptrc:: ERG12-ERG8-ERG19-IDI1 [42]
pHB1 P15A origin; CmR; PT7:: SabS1 This work
pHB2 P15A origin; CmR; PT7::IspA This work
pHB3 P15A origin; CmR; PT7::IspA-SabS1 This work
pHB4 P15A origin; CmR; PT7:: GPPS2 This work
pHB5 P15A origin; CmR; PT7:: GPPS2-SabS1 This work
pHB6 P15A origin; CmR; PT7:: mvaE-GPPS2-SabS1  
pHB7 P15A origin; CmR; PT7::mvaE-mvaS-GPPS2-SabS1 This work
E. coli BL21(DE3) E. coli B dcm ompT hsdS(rB - mB -) gal Takara
E. coli DH5α deoR, recA1, endA1, hsdR17(rk-, mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1 Invitrogen
Saccharomyces cerevisiae Type strain ATCC 204508
HB1 E. coli BL21(DE3) harboring pHB1 This work
HB2 E. coli BL21(DE3) harboring pHB3 This work
HB3 E. coli BL21(DE3) harboring pHB5 This work
HB4 E. coli BL21(DE3) harboring pHB7 and pTrcLower This work