Figure 5From: Metabolic engineering of Pseudomonas sp. strain VLB120 as platform biocatalyst for the production of isobutyric acid and other secondary metabolites Fermentation of different C18 T7 mutant strains harboring the production plasmids pPAPC-km and pPDPK. Cultivations were done in shake flasks in M9* pH 7.4 starting with 20 g L-1 glucose and 5 g L-1 yeast extract. Induction was conducted with 1 mM IPTG at OD450 of 10. Error bars indicate standard deviations (n = 2).Back to article page