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Figure 5 | Microbial Cell Factories

Figure 5

From: Autodisplay for the co-expression of lipase and foldase on the surface of E. coli: washing with designer bugs

Figure 5

Enzyme activity of whole cells displaying lipase and foldase. p-nitrophenyl palmitate was used as substrate and the increase of absorption at 405 nm was observed photometrically. The assay was performed in potassium phosphate buffer pH 7.4 at a constant temperature of 25°C. The increase in absorption is caused by the nitrophenylate anion after lipolytic cleavage of the ester bond conducted by the surface displayed lipase. = E. coli BL21(DE3)pAT-LiFoBc coexpressing both lipase and foldase ▲ = E. coli BL21(DE3) pAT-LipBc and E. coli UT5600(DE3) pAT-FoldBc mixed and preincubated for one hour ■ = E. coli BL21(DE3), host strain used as a control, ☐ = substrate solution in buffer. Mean values with standard deviations are shown, n = 3.

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