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Figure 4 | Microbial Cell Factories

Figure 4

From: Autodisplay for the co-expression of lipase and foldase on the surface of E. coli: washing with designer bugs

Figure 4

Coexpression and surface display of both lipase and foldase fusion protein. SDS-PAGE of membrane preparations of E. coli BL21(DE3)pAT-LiFoBc, coexpressing the lipase and foldase fusion protein. Molecular weight markers are indicated on the left hand side. Lane 1 shows a membrane preparation of E. coli BL21(DE3), used as a control. Lanes 2-5 show outer membrane preparations of E. coli BL21(DE3)pAT-LiFoBc. M: protein marker; IPTG: protein expression was induced by adding IPTG (final concentration: 1 mM); Proteinase K: whole cells were treated with Proteinase K, concentrations are given in mg mL-1; OmpA/OmpF: native E. coli outer membrane proteins. The foldase and lipase fusion proteins are indicated by black arrows.

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