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Figure 3 | Microbial Cell Factories

Figure 3

From: Autodisplay for the co-expression of lipase and foldase on the surface of E. coli: washing with designer bugs

Figure 3

FACS analysis of lipase surface display. Whole cells were treated with rabbit-anti-lipase-antibody and anti-rabbit Dylight coupled secondary antibody. A: host cells E. coli BL21(DE3) used as a control, B: Cells displaying another enzyme on their surface (E. coli BL21(DE3)pAT-NOx). These were used as control cells to test whether the anti-lipase-antibody binds parts of the autotransporter. C: BL21(DE3)pAT-LipBc expressing the lipase via Autodisplay. Only the reaction with BL21(DE3)pAT-LipBc and the lipase-antibody caused a tenfold increase in mean fluorescence values, which means that the lipase can be regarded as surface exposed.

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Microbial Cell Factories

ISSN: 1475-2859

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