Figure 4

Ratjadone A blocks HIV infection at a time point corresponding to viral mRNA nuclear export. TZM-bl cells were synchronously infected with HIV_LAI and plated in 96-wells plates in duplicate. Then 10 nM ratjadone A or 1 μM T20 were added at the indicated time points. The drug solvent (0.1% DMSO) was used as control. 72 h after infection luciferase activity was measured for every time point. Values are plotted as relative light units per seconds of exposure and are the mean of the duplicates. Error bars are standard error of the mean (SEM).