A comparative study: the impact of different lipid extraction methods on current microalgal lipid research

Li, Yan; Ghasemi Naghdi, Forough; Garg, Sourabh; Adarme-Vega, Tania Catalina; Thurecht, Kristofer J; Ghafor, Wael Abdul; Tannock, Simon; Schenk, Peer M

doi:10.1186/1475-2859-13-14

Microbial Cell Factories

Table 2 Comparison of normalised fatty acids (FA) composition between different extraction methods (% of dry weight) determined from FAME analysis by GC/MS

From: A comparative study: the impact of different lipid extraction methods on current microalgal lipid research

	MW	Chl:Met	Dic:Met	Pro:Hex	Eth:KOH	ScCO₂
*C14*	242	0.03 ± 0.01 (a)	0.06 ± 0.01 (ab)	0.12 ± 0.03 (b)	0.06 ± 0.02 (ab)	0.19 ± 0.01 (c)
C16	270	3.57 ± 0.14	3.63 ± 0.37	3.27 ± 0.44	3.31 ± 0.32	3.81 ± 0.47
*C16:1 (n-7)*	268	0.14 ± 0.08 (a)	0.06 ± 0.01 (a)	0.09 ± 0.02 (a)	-- (b)	0.09 ± 0.03 (a)
*C16:1*	268	0.39 ± 0.03 (a)	0.59 ± 0.05 (ab)	0.58 ± 0.12 (ab)	0.37 ± 0.06 (a)	0.81 ± 0.08 (b)
*C16:2*	266	0.06 ± 0.02 (a)	0.07 ± 0.01 (a)	0.12 ± 0.03 (a)	-- (b)	0.21 ± 0.00 (c)
C16:3	264	0.44 ± 0.05	0.36 ± 0.06	0.38 ± 0.11	0.35 ± 0.05	0.31 ± 0.02
*C18*	298	0.44 ± 0.04 (a)	0.27 ± 0.01 (b)	0.43 ± 0.05 (a)	0.34 ± 0.02 (ab)	0.38 ± 0.02 (ab)
C18:1 (n-9c)	296	0.98 ± 0.47	1.26 ± 0.13	1.17 ± 0.08	0.89 ± 0.07	1.25 ± 0.53
*C18:1 (n-9t)*	296	-- (a)	0.06 ± 0.03 (a)	0.06 ± 0.01 (a)	-- (a)	0.16 ± 0.02 (b)
C18:2 (n-6)	294	0.72 ± 0.07	0.52 ± 0.12	0.62 ± 0.07	0.68 ± 0.13	0.73 ± 0.18
*C18:3 (n-6)*	292	0.15 ± 0.03 (a)	0.16 ± 0.01 (a)	0.04 ± 0.01 (b)	0.05 ± 0.01 (b)	0.12 ± 0.03 (a)
*C18:3 (n-3)*	292	0.25 ± 0.02 (ab)	0.43 ± 0.04 (c)	0.47 ± 0.05 (c)	0.24 ± 0.07 (a)	0.41 ± 0.05 (bc)
C20	326	0.03 ± 0.02	--	--	0.06 ± 0.04	0.04 ± 0.05
*C20:1 (n-9)*	324	0.50 ± 0.06 (ab)	0.36 ± 0.03 (a)	0.47 ± 0.04 (ab)	0.37 ± 0.02 (a)	0.60 ± 0.09 (b)
*C20:4 (n-6)*	318	0.12 ± 0.00 (a)	0.20 ± 0.03 (b)	0.06 ± 0.02 (a)	0.12 ± 0.02 (a)	0.27 ± 0.03 (b)
*C20:5 (n-3)*	316	0.47 ± 0.08 (ab)	0.32 ± 0.04 (abc)	0.27 ± 0.05 (bc)	0.21 ± 0.07 (c)	0.52 ± 0.06 (a)
*C22:5 (n-3)*	344	-- (a)	-- (a)	-- (a)	-- (a)	0.03 ± 0.01 (b)
*C22:6 (n-3)*	342	-- (a)	-- (a)	0.04 ± 0.00 (b)	-- (a)	0.04 ± 0.00 (b)
Total Saturated FA (mean% of total FA)		48.65	47.43	46.98	46.83	44.36
Total Monounsaturated (mean% of total FA)		25.21	27.76	28.66	27.01	29.17
Total Polyunsaturated (mean% of total FA)		26.14	24.81	24.36	26.16	26.47
*Total FA (mean% of dry weight)*		8.33 ± 0.30 (a)	8.64 ± 0.49 (ab)	8.18 ± 0.51 (a)	6.06 ± 0.44 (c)	10.00 ± 0.27 (b)

Chl:Met -- chloroform and methanol method; Dic:Met -- dichloromethane and methanol method; Pro:Hex -- propan-2-ol and cyclohexane method; Eth:KOH -- ethanol and KOH method; ScCO₂ -- supercritical-CO₂ extraction method.
Notes: Values less than 0.03% were deleted from the calculation to eliminate the effect of background and labelled as “--“ in the table. The Bold and Italic parameters in the first column were dependent on extraction method, indicated by different small letters in brackets which show significant differences between extractions methods (P < 0.05).

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ISSN: 1475-2859

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