Table 3 Primers used in this article
Primer | Sequence (5′-3′) | Description | Expected size (bp) |
|---|---|---|---|
OriTF | GCCAGCTCGTCGGTGTAGC | OriT | 700 |
OriTR | CAACAACGTTGCCCGGATCG | ||
ZF | TGCACTGCAGCAAGACACTGTGAAGGC | RepA and its flanking sequences | 2100 |
ZR | GATTCATTATAACCACGGATCCCTGCCTTTAAGATTTG | ||
EmCFB | CAAATCTTAAAGGCAGGGATCCGTGGTTATAATGAATC | EmR in pOriT-4Em | 825 |
EmC | GGACTAGTGTTAAGGGATGCAGTTTATG | ||
UPF | ACGCGTCGACGTATGGTGCTGCTGATAACAGC | Upstream homologous fragment of epsT | 834 |
UPR | CGCGGATCCCAGTGTGAGTAGCACCTCAC | ||
DnF | CGGGATCCGTGAAAATGCGTACTAA | Downstream homologous fragment of epsT | 830 |
DnR | CATTATAACCACTCTAGACCACTAAAGTTATCG | ||
EmF | CGATAACTTTAGTGGTCTAGAGTGGTTATAATG | EmR in pMT | 825 |
EmR | CTAGCTAGCGTTAAGGGATGCAGTTTATG | ||
sacbF | CATGCCATGGCACATATACCTGCCGTTCAC | sacB | 1900 |
sacBR | CCGGGCCCAATGCCAATAGGATATCGGC | ||
TyzF | GACGATGAATGGAGTGGTAAGATAG | Confirmation of the second recombination | 3400a |
TyzR | CTCCAATCATGCTGCCATGTTGC | 2000b |