Table 3 Overview of extracellular acid concentrations (mM) measured in time during fermentation
From: Expression of the Aspergillus terreus itaconic acid biosynthesis cluster in Aspergillus niger
Citric acid | Itaconic acid | cis-aconitic acid | Succinic acid | Malic acid | Pyruvic acid | α-ketoglutaric acid | ||
|---|---|---|---|---|---|---|---|---|
6 h | sCAD4 | 3.9 | ND | ND | ND | ND | ND | ND |
cadA + mfsA 2.5 | 4.0 | ND | ND | ND | ND | ND | ND | |
cadA + mttA 1.2 | 2.7 | 4.0 | ND | 0.2 | ND | ND | ND | |
cadA + mttA + mfsA 4 | 3.3 | 2.7 | ND | ND | ND | ND | ND | |
30 h | sCAD4 | 1.8 | 1.45 | 0.3 | ND | ND | 2.4 | ND |
cadA + mfsA 2.5 | 8.5 | 0.6 | ND | ND | ND | ND | ND | |
cadA + mttA 1.2 | 2.5 | 28.1 | ND | 0.7 | ND | ND | 1.5 | |
cadA + mttA + mfsA 4 | 1.9 | 21.1 | 1.2 | ND | ND | 0.8 | ND | |
54 h | sCAD4 | 1.9 | 1.7 | 0.3 | ND | ND | 2.1 | ND |
cadA + mfsA 2.5 | 11.1 | 0.8 | ND | 0.8 | 0.7 | ND | ND | |
cadA + mttA 1.2 | 1.8 | 46.0 | ND | 1.4 | ND | ND | 0.4 | |
cadA + mttA + mfsA 4 | 1.8 | 43.6 | 1.8 | ND | ND | 0.6 | ND | |
78 h | sCAD4 | 2.3 | 2.0 | 0.3 | ND | ND | 2.2 | ND |
cadA + mfsA 2.5 | 12.7 | 1.0 | ND | 0.9 | 0.7 | ND | ND | |
cadA + mttA 1.2 | 1.5 | 41.1 | ND | 0.9 | ND | ND | ND | |
cadA + mttA + mfsA 4 | 1.8 | 54.3 | 2.2 | 0.9 | ND | ND | ND |