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Figure 5 | Microbial Cell Factories

Figure 5

From: Rational selection and engineering of exogenous principal sigma factor (σHrdB) to increase teicoplanin production in an industrial strain of Actinoplanes teichomyceticus

Figure 5

Schematic mapping of the best chimera P-22 and mutation sites introduced by error-prone PCR in the isolated σ HrdB of the recombinants S-17 and S-45. Conserved regions were shown in both the parent σHrdB and its evolved offspring. Triple parental contribution in region 1.1 of the hybrid was manifested (gray background, box and underline presented parts of σHrdB amino acid residues in Salinispora arenicola CNS-205, Actinoplanes missouriensis 431 and Micromonospora aurantiaca ATCC 27029 respectively), in which the asterisk labeled amino acids were obtained during the StEP process. Other regions of the chimera harbored the same amino acid residues as those in HrdB of Actinoplanes missouriensis 431.

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Microbial Cell Factories

ISSN: 1475-2859

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