Table 2 Kinetic parameters of Est_p6 for various pNP esters
Substrate (pNP ester) | K m(mM) | V max(μmol∙min-1∙mg-1) | k cat(s-1) | k cat/K m(s-1 mM-1) |
|---|---|---|---|---|
Acetate (C2) | 2.644 | 212.695 | 133.303 | 50.413 |
Butyrate (C4) | 1.148 | 3496.626 | 2191.452 | 1908.445 |
Caprylate (C8) | 0.322 | 914.645 | 573.239 | 1779.330 |
Caprate (C10) | 1.280 | 163.068 | 102.200 | 79.815 |
Laurate (C12) | 0.149 | 0.795 | 0.499 | 3.339 |