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Figure 2 | Microbial Cell Factories

Figure 2

From: Isolation of a novel alkaline-stable lipase from a metagenomic library and its specific application for milkfat flavor production

Figure 2

Purification of recombinant Est_p6. Proteins recovered during various purification steps as described in the text were separated by SDS-10% polyacrylamide gel electrophoresis and stained with Coomassie Brilliant Blue R-250. Lane M, molecular weight standards (kDa values indicated at left); Lane 1, total cell lysate; Lane 2, soluble fragment of cell lysate; Lane 3, flow-through fraction; Lane 4, 250 mM imidazole elution fraction. Purified Est_p6 was indicated by the triangle pointer.

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Microbial Cell Factories

ISSN: 1475-2859

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