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Figure 1 | Microbial Cell Factories

Figure 1

From: Isolation of a novel alkaline-stable lipase from a metagenomic library and its specific application for milkfat flavor production

Figure 1

Bioinformatic analysis of Est_p6. (A) Unrooted neighbor-joining phylogenetic tree of Est_p6 (red triangle) and related bacterial lipolytic enzymes, based on conserved sequence motifs. The a.a. sequences of the other enzymes were obtained from published data. Sequence alignment was performed using ClustalW version 2.0, and the tree was created using MEGA version 5.2. Scale bar: number of a.a. substitutions per site. (B) Conserved sequence blocks from multiple sequence alignment between Est_p6 and family IV members. Sequence alignment was performed using ClustalW and ESPript. Conserved sequences are indicated by boxes, and similar sequences are indicated by colored background. The catalytic triads (red triangles) and the typical motif of family IV (pink circles) are identical.

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