Table 4 Densitometric analysis of dot blot experiments with the pellets of the adsorption reaction with wild type and cotH mutant spores
LTB source | Amount of sample used | Density (OD/mm2) | Amount of LTB (ng) | Amount of LTB (μg/2.0 x 109spore) (% total) |
|---|---|---|---|---|
Purified LTB | 25.0 ng | 75.553 | NA | NA |
12.5 ng | 40.385 | NA | NA | |
6.25 ng | 22.113 | NA | NA | |
wt | 1 x 107 | 38.631 | 8.2 | 1.64 (82) |
5 x 106 | 15.227 | 3.5 | 1.41 (70) | |
2.5 x 106 | 9.238 | 1.6 | 1.29 (65) | |
cotH | 5 x 106 | 18.527 | 4.7 | 1.90 (95) |
2.5 x 106 | 7.857 | 2.2 | 1.836 (91) | |
1.25 x 106 | 3.864 | 1.1 | 1.888 (94) |