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Table 3 Densitometric analysis of dot blot experiments with the supernatants of the adsorption reaction with wild type and cotH mutant spores

From: Non-recombinant display of the B subunit of the heat labile toxin of Escherichia coli on wild type and mutant spores of Bacillus subtilis

LTB source

Amount of sample used

Density (OD/mm2)

Amount of LTB (ng)

Amount of LTB μg in 200 μl (% total)

Purified LTB

12.50 ng

25.125

NA

NA

 

6.25 ng

10.875

NA

NA

 

3.12 ng

4.621

NA

NA

wt

40 μl

16.517

9.2

0.04 (2.0)

 

20 μl

8.361

4.1

0.04 (2.1)

 

10 μl

4.383

1.9

0.03 (1.9)

cotH

80 μl

7.522

3.8

0.009 (0.4)

 

40 μl

2.987

1.2

0.006 (0.3)