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Figure 6 | Microbial Cell Factories

Figure 6

From: Non-recombinant display of the B subunit of the heat labile toxin of Escherichia coli on wild type and mutant spores of Bacillus subtilis

Figure 6

Immunofluorescence microscopy with anti-GM1 antibody. Wild type, cotH mutant and recombinant cotC::eltB spores were adsorbed with 10.0 μg of LTB, treated with GM1 and reacted with anti-GM1 antibody and Texas Red conjugated secondary antibody. Spores adsorbed with LTB but not treated with GM1 were also reacted with GM1 antibody (negative control). A representative microscopy field for each strain is observed by phase contrast and immunofluorescence. Merged panels of phase contrast and immunofluorescence are shown. Exposure times were typically in the range of 50–200 ms.

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