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Figure 5 | Microbial Cell Factories

Figure 5

From: Non-recombinant display of the B subunit of the heat labile toxin of Escherichia coli on wild type and mutant spores of Bacillus subtilis

Figure 5

Saturation of LTB adsorption. (A) The adsorption reactions were performed with 2.0 × 109 spores of the wild type and cotH mutant strain and with 2.0, 10.0 and 20.0 μg of purified LTB. Serial dilutions of purified LTB were used as a standard. Wild type and mutant spores not adsorbed with LTB were used as negative controls. Immuno-reactions were performed with anti-LTB antibody [10] and anti-rabbit secondary antibody conjugated with the horseradish peroxidase. (B) Percentage of LTB bound to the spore surface of wild type (gray bars) or cotH mutant (black bars) spores. The amount of LTB used in the adsorption reaction was considered as 100%. Error bars represent the standard deviation of each experiments.

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