High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli

Table 1 Growth and pH characteristics of different E. coli cultures

Strain	Medium	OD₆₀₀at induction	pH at induction	OD₆₀₀at harvest	pH at harvest	CDW (g/L) at harvest
SHuffle® T7 Express [pET-28b:7C12]	LB	1.0	7.3	1.4	8.8	0.38
	TB	1.1	7.2	8.3	8.4	2.24
	EnPresso	10.6	6.5	13.1	6.9	3.54
SHuffle® T7 Express [pET-28b:EG2]	LB	1.0	7.3	1.8	8.7	0.49
SHuffle® T7 Express [pET-28b:EG2]	TB	1.0	7.1	10.7	8.1	2.89
	EnPresso	10.2	6.6	19.8	7.0	5.35

Samples from each cultivation were taken at the time point of induction and harvest and subsequently, OD₆₀₀ and pH were determined. Cell dry weight (CDW) was calculated at the end of cultivation, whereas one unit of OD₆₀₀ corresponds to a dry cell weight of 0.27 g/L [52].

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