Figure 6
Radio-TLC, SDS-PAGE and autoradiography analysis of99mTc-labeled sdAbs. After radiolabeling of 7C12 at its carboxyterminal hexahistidine epitope with [99mTc(H2O)3(CO)3]+, the radiochemical yield was determined by radio-TLC (A). Identical graphs were obtained for EG2 and are therefore not shown. 99mTc-sdAbs were further analyzed by SDS-PAGE (B) and autoradiography (C) in the absence of DTT. The protein contents were visualized by Coomassie staining. Radiolabeling, SDS-PAGE and autoradiography analysis were repeated twice and representative pictures are shown.