Skip to main content
Figure 5 | Microbial Cell Factories

Figure 5

From: High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli

Figure 5

Analysis of purified sdAbs under reducing and non-reducing conditions. Ten μg of purified sdAbs 7C12 (lane 1 and 3) and EG2 (lane 2 and 4) generated in E. coli SHuffle® T7 Express were analyzed by SDS-PAGE in the absence and presence of the reducing agent dithiothreitol (DTT) with subsequent Coomassie staining (A) or immunological detection using a HRP-conjugated Penta · His antibody directed against the hexahistidine epitope (B). A weak band corresponding to sdAb dimer is highlighted by an arrow. SDS-PAGE and immunostaining were repeated twice and representative pictures are shown.

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us