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Figure 3 | Microbial Cell Factories

Figure 3

From: High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli

Figure 3

Recombinant protein production of recombinant E. coli strains SHuffle® T7 Express [pET-28b:7C12] and [pET-28b:EG2] using different cultivation media. Total and soluble protein fractions of recombinant strains at 24 h after induction separated by SDS-PAGE with subsequent Coomassie staining (A) or immunological detection using an HRP-conjugated Penta His antibody directed against the hexahistidine epitope (B). In order to allow direct comparison between different cultivation media, all samples were diluted depending to their optical density to equal cell concentration before cell lysis and gel loading. LB = Luria-Bertani broth, TB = Terrific broth, EN = EnPresso medium, T = total cell lysate, S = soluble fraction of cell lysate. The experiments were repeated twice and representative pictures are shown.

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Microbial Cell Factories

ISSN: 1475-2859

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