Figure 2

Recorded data during cultivation of recombinant E. coli strain SHuffle® T7 Express [pET-28b:7C12] in different culture media. Cell density (A) and pH (B) measurements for EnPresso medium (EN), Luria-Bertani medium (LB) and Terrific Broth (TB). The time point of induction (1 mM IPTG) is highlighted with a dashed vertical line. Cultures in LB and TB were grown for 6 h at 30°C before induction, whereas EnPresso cultures were grown for 24 h at 30°C before induction. All cultures were harvested 24 h after induction. The cultivation experiment was repeated twice and average values are shown.