Figure 4
From: Selective steroid oxyfunctionalisation by CYP154C5, a bacterial cytochrome P450
Turnover number (TON) determination for whole cells of E. coli C43 (DE3) (pIT2cyp154C5) (pACYCcamAB) in steroid biotransformations. TTN and conversion of a) pregnenolone (1) and b) progesterone (3) and testosterone (5) over time are given. All bioconversions were carried out in 50 mM potassium phosphate buffer pH 7.4 at 30°C with an OD600 of 40 and 30 mM glucose for cofactor regeneration. Substrates (2 mM) were added as stock solutions in 36% w/v hydroxypropyl-β-cyclodextrin in water. Reactions were performed in triplicate.