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Figure 4 | Microbial Cell Factories

Figure 4

From: Construction of an in vitro bypassed pyruvate decarboxylation pathway using thermostable enzyme modules and its application to N-acetylglutamate production

Figure 4

Acetyl-CoA production through the bypassed pyruvate decarboxylation pathway. Heat-treated cells of recombinant E. coli having Ap PDC and Tt ADDH (approximately 8 and 4 mg ml-1, respectively) were incubated at 50°C with 2 mM each of pyruvate, NAD+, and CoA. Acetyl-CoA (green circle), CoA (yellow square) and acetate (blue diamond) concentrations were quantified by HPLC.

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Microbial Cell Factories

ISSN: 1475-2859

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