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Figure 3 | Microbial Cell Factories

Figure 3

From: Construction of an in vitro bypassed pyruvate decarboxylation pathway using thermostable enzyme modules and its application to N-acetylglutamate production

Figure 3

Thermal stability of Ap PDC (a), Tt ADDH (b), Tt GDH (c), and Tm NAGS (d). Crude lysates of the recombinant E. coli were preheated at 70°C for 30 min; an incubation temperature of 60°C was employed for preheating the lysate having Ap PDC. After the removal of denatured proteins by centrifugation, the enzyme solutions were incubated at 50°C (blue diamond), 60°C (yellow square), and 70°C (green triangle) for the indicated time periods. Residual enzyme activity was determined using the standard assay conditions.

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Microbial Cell Factories

ISSN: 1475-2859

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