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Figure 2 | Microbial Cell Factories

Figure 2

From: Construction of an in vitro bypassed pyruvate decarboxylation pathway using thermostable enzyme modules and its application to N-acetylglutamate production

Figure 2

Effect of CoA concentrations on the reaction specificity of Tt ADDH. The reaction was performed at 50°C in the mixture composed of 50 mM HEPES-NaOH (pH 7.0), 1 mM NAD+, 1 mM acetaldehyde, approximately 0.16 U ml-1Tt ADDH, and the indicated concentrations of CoA. After the preincubation for 1 min at 50°C, the reaction was initiated by the addition of acetaldehyde. After the incubation at 50°C for 1 min, the reaction was quenched by adding an equal volume of ice-cold 0.5 N HCl in methanol. The mixture was analyzed by HPLC to quantify acetate (green bar) and acetyl-CoA (yellow bar).

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