Strain, plasmid, primer, or transformant | Relevant features | Source or reference |
---|---|---|
Strain | ||
Escherichia coli strains | ||
Nova blue | endA1 hsdR17(r K12 -m K12 +) supE44 thi-I gyrA96 relA1 lac | Novagene |
recA1/F’[proAB + lacIq ZΔM15::Tn10(Tetr)] | ||
Streptomyces lividans 1326 | WT strain | NBRC |
Plasmids | ||
pUC702-pro-sig-term | Versatile vector for protein expression; thiostrepton resistance marker | [15] |
pUC702-plds-bgl | Vector for secreting β-glucosidase(Tfu0937) using plds; thiostrepton resistance marker | [15] |
pUC702-xcs-bgl | Vector for secreting β-glucosidase(Tfu0937) using xcs; thiostrepton resistance marker | This study |
pCU702-tfu0620s-bgl | Vector for secreting β-glucosidase(Tfu0937) using tfu0620s; thiostrepton resistance marker | This study |
pUC702-tfu2486s-bgl | Vector for secreting β-glucosidase(Tfu0937) using tfu2486s; thiostrepton resistance marker | This study |
pUC702-tfu2176s-bgl | Vector for secreting β-glucosidase(Tfu0937) using tfu2716s; thiostrepton resistance marker | This study |
pUC702-tfu1612s-bgl | Vector for secreting β-glucosidase(Tfu0937) using tfu1612s; thiostrepton resistance marker | This study |
pUC702-tfu2788s-bgl | Vector for secreting β-glucosidase(Tfu0937) using tfu2788s; thiostrepton resistance marker | This study |
pTYM18 | Intergeneric conjugation vector; kanamycin resistance marker | [23] |
pTYM18-xcs-bgl | Vector for secreting β-glucosidase(Tfu0937) using xcs; kanamycin resistance marker | This study |
pTYM18-plds-bgl | Vector for secreting β-glucosidase(Tfu0937) using xcs; kanamycin resistance marker | This study |
Transformants | ||
S. lividans/pU-plds-bgl | Transformant harboring pUC702-plds-β-glucosidase (Tfu0937) | [15] |
S. lividans/pU-xcs-bgl | Transformant harboring pUC702-xcs-β-glucosidase (Tfu0937) | This study |
S. lividans/pU-0620 s-bgl | Transformant harboring pUC702-tfu0620s-β-glucosidase (Tfu0937) | This study |
S. lividans/pU-2486 s-bgl | Transformant harboring pUC702-tfu2486s-β-glucosidase (Tfu0937) | This study |
S. lividans/pU-2176 s-bgl | Transformant harboring pUC702-tfu2176s-β-glucosidase (Tfu0937) | This study |
S. lividans/pU-1612 s-bgl | Transformant harboring pUC702-tfu1612s-β-glucosidase (Tfu0937) | This study |
S. lividans/pU-2788 s-bgl | Transformant harboring pUC702-tfu2788s-β-glucosidase (Tfu0937) | This study |
S. lividans/pT-xcs-bgl | Transformant integrated pTYM18-xcs-β-glucosidase (Tfu0937) | This study |
S. lividans/pTxcs-pUxcs | Transformant integrated pTYM18-xcs-β-glucosidase (Tfu0937) and harboring pUC702-xcs-β-glucosidase (Tfu0937) | This study |
S. lividans/pTxcs-pUplds | Transformant integrated pTYM18-xcs-β-glucosidase (Tfu0937) and harboring pUC702-plds-β-glucosidase (Tfu0937) | This study |
Oligonucleotide primers | ||
xcs_Fw | aGCATGCatgcagcaggacggcacacagcagg | |
xcs_Rv | tttGCTAGCggcgtgggctgtgccgggcagcagc | |
tfu0620s_Fw | TCGTTTAAGGATGCAatgagtaaagttcgtgccacgaaca | |
tfu0620s_Rv | CGATTGCGAGGTCACggcggcgttggccggagcagcgaac | |
tfu2486s_Fw | TCGTTTAAGGATGCAatgtttcgacgtctgcctgtgctgg | |
tfu2486s_Rv | CGATTGCGAGGTCACtgccgcctgagcgtccacgtcagcg | |
tfu2176s_Fw | TCGTTTAAGGATGCAatgtccgtcactgaacctcctcccc | |
tfu2176s_Rv | CGATTGCGAGGTCACttcggcgtgggcggttcccgtggcc | |
tfu1612s_Fw | TCGTTTAAGGATGCAatgacagcaacagcacagcgaacac | |
tfu1612s_Rv | CGATTGCGAGGTCACggcggcagcggagtggacgaggccg | |
Tfu2788s_Fw | TCGTTTAAGGATGCAatgtccgtcactgaacctcctcccc | |
Tfu2788s_Rv | CGATTGCGAGGTCACttcggcgtgggcggttcccgtggcc |