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Table 2 Strains, plasmids, transformants, and oligonucleotide primers used in this study

From: Hyper secretion of Thermobifida fusca β-glucosidase via a Tat-dependent signal peptide using Streptomyces lividans

Strain, plasmid, primer, or transformant Relevant features Source or reference
Strain   
Escherichia coli strains   
Nova blue endA1 hsdR17(r K12 -m K12 +) supE44 thi-I gyrA96 relA1 lac Novagene
  recA1/F’[proAB + lacIq ZΔM15::Tn10(Tetr)]  
Streptomyces lividans 1326 WT strain NBRC
Plasmids   
pUC702-pro-sig-term Versatile vector for protein expression; thiostrepton resistance marker [15]
pUC702-plds-bgl Vector for secreting β-glucosidase(Tfu0937) using plds; thiostrepton resistance marker [15]
pUC702-xcs-bgl Vector for secreting β-glucosidase(Tfu0937) using xcs; thiostrepton resistance marker This study
pCU702-tfu0620s-bgl Vector for secreting β-glucosidase(Tfu0937) using tfu0620s; thiostrepton resistance marker This study
pUC702-tfu2486s-bgl Vector for secreting β-glucosidase(Tfu0937) using tfu2486s; thiostrepton resistance marker This study
pUC702-tfu2176s-bgl Vector for secreting β-glucosidase(Tfu0937) using tfu2716s; thiostrepton resistance marker This study
pUC702-tfu1612s-bgl Vector for secreting β-glucosidase(Tfu0937) using tfu1612s; thiostrepton resistance marker This study
pUC702-tfu2788s-bgl Vector for secreting β-glucosidase(Tfu0937) using tfu2788s; thiostrepton resistance marker This study
pTYM18 Intergeneric conjugation vector; kanamycin resistance marker [23]
pTYM18-xcs-bgl Vector for secreting β-glucosidase(Tfu0937) using xcs; kanamycin resistance marker This study
pTYM18-plds-bgl Vector for secreting β-glucosidase(Tfu0937) using xcs; kanamycin resistance marker This study
Transformants   
S. lividans/pU-plds-bgl Transformant harboring pUC702-plds-β-glucosidase (Tfu0937) [15]
S. lividans/pU-xcs-bgl Transformant harboring pUC702-xcs-β-glucosidase (Tfu0937) This study
S. lividans/pU-0620 s-bgl Transformant harboring pUC702-tfu0620s-β-glucosidase (Tfu0937) This study
S. lividans/pU-2486 s-bgl Transformant harboring pUC702-tfu2486s-β-glucosidase (Tfu0937) This study
S. lividans/pU-2176 s-bgl Transformant harboring pUC702-tfu2176s-β-glucosidase (Tfu0937) This study
S. lividans/pU-1612 s-bgl Transformant harboring pUC702-tfu1612s-β-glucosidase (Tfu0937) This study
S. lividans/pU-2788 s-bgl Transformant harboring pUC702-tfu2788s-β-glucosidase (Tfu0937) This study
S. lividans/pT-xcs-bgl Transformant integrated pTYM18-xcs-β-glucosidase (Tfu0937) This study
S. lividans/pTxcs-pUxcs Transformant integrated pTYM18-xcs-β-glucosidase (Tfu0937) and harboring pUC702-xcs-β-glucosidase (Tfu0937) This study
S. lividans/pTxcs-pUplds Transformant integrated pTYM18-xcs-β-glucosidase (Tfu0937) and harboring pUC702-plds-β-glucosidase (Tfu0937) This study
Oligonucleotide primers   
xcs_Fw aGCATGCatgcagcaggacggcacacagcagg  
xcs_Rv tttGCTAGCggcgtgggctgtgccgggcagcagc  
tfu0620s_Fw TCGTTTAAGGATGCAatgagtaaagttcgtgccacgaaca  
tfu0620s_Rv CGATTGCGAGGTCACggcggcgttggccggagcagcgaac  
tfu2486s_Fw TCGTTTAAGGATGCAatgtttcgacgtctgcctgtgctgg  
tfu2486s_Rv CGATTGCGAGGTCACtgccgcctgagcgtccacgtcagcg  
tfu2176s_Fw TCGTTTAAGGATGCAatgtccgtcactgaacctcctcccc  
tfu2176s_Rv CGATTGCGAGGTCACttcggcgtgggcggttcccgtggcc  
tfu1612s_Fw TCGTTTAAGGATGCAatgacagcaacagcacagcgaacac  
tfu1612s_Rv CGATTGCGAGGTCACggcggcagcggagtggacgaggccg  
Tfu2788s_Fw TCGTTTAAGGATGCAatgtccgtcactgaacctcctcccc  
Tfu2788s_Rv CGATTGCGAGGTCACttcggcgtgggcggttcccgtggcc