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Figure 2 | Microbial Cell Factories

Figure 2

From: Hyper secretion of Thermobifida fusca β-glucosidase via a Tat-dependent signal peptide using Streptomyces lividans

Figure 2

The secretory production of BGL using signal peptides screened in this study. (A) Time-course of culture supernatant β-glucosidase activity: S. lividans/pU-xcs-bgl (closed circles); S. lividans/pU-0620 s-bgl (closed squares); S. lividans/pU-2486 s-bgl (closed diamonds); S. lividans/pU-plds-bgl (closed triangles) in modified TSB medium with 5% tryptone and 3% glucose. Each data point shows the average of 3 independent experiments, and error bars represent the standard deviation. (B) Time-course of dry cell weight: S. lividans/pU-xcs-bgl (open circles); S. lividans/pU-0620 s-bgl (open squares); S. lividans/pU-2486 s-bgl (open diamonds); S. lividans/pU-plds-bgl (open triangles) in modified TSB medium with 5% tryptone and 3% glucose. Each data point shows the average of 3 independent experiments, and error bars represent the standard deviation.

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