Engineering glutathione biosynthesis of Saccharomyces cerevisiae increases robustness to inhibitors in pretreated lignocellulosic materials

Table 4 Results from SSF of pretreated spruce with the strains constructed in the present study

Strain	Ethanol yield on hexoses^a(g g^-1)	Ethanol concentration at 48 h (g L^-1)
CEN.PK 113-7D	0.10 ± 0.00	8.2 ± 0.0
GSH1	0.16 ± 0.00	13.7 ± 0.2
GSH1/CYS3	0.16 ± 0.00	13.1 ± 0.3
GLR1	0.07 ± 0.00	6.0 ± 0.0
GSH1/GLR1	0.17 ± 0.01	14.0 ± 1.0
GSH1/CYS3/GLR1	0.14 ± 0.00	12.3 ± 0.2

^aThe ethanol yield was calculated based on the total amount of hexoses present in the solid and liquid fraction of the pretreated raw material at the start of the SSF.
The values given are mean values from duplicate cultivations ± standard deviation.

ISSN: 1475-2859