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Table 2 Comparative data from 1 L batch fermentations of strains AR26 and AR36, using 100 g/L of Glc and 15 g/L of YE as substrates

From: Constitutive expression of selected genes from the pentose phosphate and aromatic pathways increases the shikimic acid yield in high-glucose batch cultures of an Escherichia coli strain lacking PTS and pykF

Strain

AR26

AR36

SA titer (g/L)

12.95 ± 0.64

41.80 ± 2.83

Glc consumed (g/L)

82.65 ± 4.88

103.70 ± 6.79

Duration of culture (h)

47

60

Y SA/Glc (mol/mol)

0.16 ± 0.02

0.42 ± 0.00

Acetate titer (g/L)

29.35 ± 0.21

11.90 ± 0.14

X max (g/L)

6.18 ± 0.10

6.54 ± 0.09

μ (h -1 )

0.45 ± 0.01

0.45 ± 0.02

Qp global (gSA/L*h)

0.27 ± 0.02

0.75 ± 0.07

Qs global (gGlc/L*h)

-1.76 ± 0.10

-1.73 ± 0.11

  1. YSA/Glc = SA yield from Glc; Xmax = maximum attained biomass expressed as dry cell weight; Qpglobal = volumetric SA productivity for the entire fermentation; Qsglobal = volumetric Glc consumption rate for the entire fermentation; μ = specific growth rate.