Table 1 Escherichia coli strains and plasmids utilized in this report

JM101

F´ tra D36 proA ⁺ proB ⁺ lacI q lac ZΔM15/supE thi Δ(lac-proAB) rpoS(33 am)

Messing [41]

PB11

JM101 Δ(ptsH, ptsI, crr)::kan

Flores et al. [21]; Flores et al. [22]

PB12

PB11, PTS^- Glc⁺; laboratory-evolved strain

Flores et al. [21]; Flores et al. [22]

AR2

PB12 lacI^-aroK^-aroL^-

This work

AR3

PB12 lacI^-aroK^-aroL^-pykF^-

This work

AR26

AR2 + pTrcAro6 (Trc/aroB⁺tktA⁺aroGfbr⁺aroE⁺aroD⁺zwf⁺)

This work

AR36

AR3 + pTrcAro6 (Trc/aroB⁺tktA⁺aroGfbr⁺aroE⁺aroD⁺zwf⁺)

This work

AR2e

AR2 + pTrc327par (plasmid vector without synthetic operon)

This work

AR3e

AR3 + pTrc327par (plasmid vector without synthetic operon)

This work

Name

Characteristics

Reference

pKD3

PCR template for amplification of chloramphenicol resistance gene flanked by homologous sequences

Datsenko and Wanner [42]

pKD46

Plasmid expressing λ-Red recombinase system with thermosensitive origin of replication

Datsenko and Wanner [42]

pCP20

FLP recombinase expression plasmid

Cherepanov and Wackernagel [43]

pBR327par

Derivative of pBR322 exhibiting increased copy number and segregational stability

Zurita et al. [32]

pTrc99A

Multipurpose expression plasmid bearing lacI gene and a polylinker in front of Trc promoter

Amann et al. [31]

pTrc327par

Contains the promoter, polylinker, and terminators of pTrc99A, and par and ori regions of pBR327par

This work (Figure 2)

pTrcAro6

pTrc327par containing a 6-gene synthetic operon to enhance the production of shikimate

This work (Figure 2)