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Figure 2 | Microbial Cell Factories

Figure 2

From: Constitutive expression of selected genes from the pentose phosphate and aromatic pathways increases the shikimic acid yield in high-glucose batch cultures of an Escherichia coli strain lacking PTS and pykF

Figure 2

Simplified scheme of the steps required in the construction of plasmid pTrcAro6, carrying 6 synthetic genes under the control of the Trc promoter. First, a segment of pTrc99A was amplified and ligated into pBR327par, creating plasmid pTrc327par. A synthetic operon comprising the required genes (aroB, tktA, aroGfbr, aroE, aroD, and zwf) was assembled separately and transferred to pTrc327par, generating pTrcAro6. The dotted lines indicate the site and orientation of some of the performed ligation reactions. Only the relevant restriction sites are displayed. A more detailed scheme of the constructions is presented in Additional file 3.

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Microbial Cell Factories

ISSN: 1475-2859

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