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Figure 1 | Microbial Cell Factories

Figure 1

From: Constitutive expression of selected genes from the pentose phosphate and aromatic pathways increases the shikimic acid yield in high-glucose batch cultures of an Escherichia coli strain lacking PTS and pykF

Figure 1

Genetic modifications applied in this report to enhance the production of shikimic acid from glucose in the laboratory-evolved E. coli strain PB12. Inactivated genes are indicated with a cross and plasmid-expressed genes are circled (see Methods for details). Dashed arrows indicate more than one catalytic step. G6P = glucose 6-phosphate; F6P = fructose 6-phosphate; GAP = glyceraldehyde 3-phosphate; 6PGNL = 6-phosphogluconolactone; Ru5P = ribulose 5-phosphate; R5P = ribose 5-phosphate; Xu5P = xylulose 5-phosphate; S7P = sedoheptulose 7-phosphate; E4P = erythrose 4-phosphate; PEP = phosphoenolpyruvate; PYR = pyruvate; ACoA = acetyl-coenzyme A; Ace-P = acetyl phosphate; CIT = citrate; OAA = oxaloacetate; DAHP = 3-deoxy-D-arabinoheptulosonate 7-phosphate; DHQ = 3-dehydroquinic acid; DHS = 3-dehydroshikimic acid; QA = quinic acid; GA = gallic acid; SA = shikimic acid; S3P = shikimate 3-phosphate; CHO = chorismate; IICBGlc = membrane component of glucose-specific PTS permease; E1 = PTS enzyme 1; Hpr = PTS histidine protein; IIAGlc = cytosolic component of glucose-specific PTS permease. Genes coding for enzymes not named in the figure: galP, galactose permease; glk, glucokinase; pgi, phosphoglucose isomerase; pfkA, 6-phosphofructokinase I; fbaA, fructose bisphosphate aldolase class II; gapA, glyceraldehyde 3-phosphate dehydrogenase; eno, enolase; actP, acetate permease; acs, acetyl-coenzyme A synthetase; pta, phosphate acetyltransferase; ackA, acetate kinase; poxB, pyruvate oxidase.

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Microbial Cell Factories

ISSN: 1475-2859

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