Table 4 cMCSs for S. cerevisiae : number of remaining elementary modes (EMs), minimum and maximum IPP carbon yield as well as maximum biomass carbon yield on glucose [C-mol/C-mol] for different knockout strategies
From: In silico profiling of Escherichia coli and Saccharomyces cerevisiae as terpenoid factories
cMCS knockout strategy | EMs | Min. IPP yield | Max. IPP yield | Max. biomass yield |
|---|---|---|---|---|
Wild type | 9,844 | - | 0.53 | 0.80 |
Strategy 1: acetate ex + {ethanol ex OR alcohol DH} + {α-ketoglutarate DH OR succinyl-CoA ligase} | 142/48 | 0.33 | 0.53 | 0.29 |
Strategy 2: acetate ex + {ethanol ex OR alcohol DH} + malate DH + malic enzyme | 60/21 | 0.33 | 0.53 | 0.29 |
Strategy 3: acetate ex + {ethanol ex OR alcohol DH} + malate DH + NAD+-dependent isocitrate DH + pyruvate DH complex | 51/19 | 0.27 | 0.53 | 0.37 |
Wild type plus DXP pathway | 11,738 | - | 0.64 | 0.80 |
Strategy 1: glucose-6-phosphate-isomerase | 631 | 0.37 | 0.63 | 0.35 |