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Table 4 cMCSs for S. cerevisiae : number of remaining elementary modes (EMs), minimum and maximum IPP carbon yield as well as maximum biomass carbon yield on glucose [C-mol/C-mol] for different knockout strategies

From: In silico profiling of Escherichia coli and Saccharomyces cerevisiae as terpenoid factories

cMCS knockout strategy EMs Min. IPP yield Max. IPP yield Max. biomass yield
Wild type 9,844 - 0.53 0.80
Strategy 1: acetate ex + {ethanol ex OR alcohol DH} + {α-ketoglutarate DH OR succinyl-CoA ligase} 142/48 0.33 0.53 0.29
Strategy 2: acetate ex + {ethanol ex OR alcohol DH} + malate DH + malic enzyme 60/21 0.33 0.53 0.29
Strategy 3: acetate ex + {ethanol ex OR alcohol DH} + malate DH + NAD+-dependent isocitrate DH + pyruvate DH complex 51/19 0.27 0.53 0.37
Wild type plus DXP pathway 11,738 - 0.64 0.80
Strategy 1: glucose-6-phosphate-isomerase 631 0.37 0.63 0.35
  1. DH = dehydrogenase; ex = excretion.