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Table 3 cMCSs for E. coli: number of remaining elementary modes (EMs), minimum and maximum IPP yield as well as maximum biomass yield on glucose or glycerol [C-mol/C-mol] for different knockout strategies

From: In silico profiling of Escherichia coli and Saccharomyces cerevisiae as terpenoid factories

cMCS knockout strategy

EMs

Min. IPP yield

Max. IPP yield

Max. biomass yield

Wild type on glucose

36,590

-

0.67

0.85

Strategy 1: O2 uptake + {lactate ex OR lactate formation} + {ethanol ex OR ethanol formation OR acetaldehyde formation} + {succinate ex OR succinate OR malate DH OR fumarate hydratase}

60-86

0.38

0.48

0.08

Strategy 2: transhydrogenase + {phosphoglycerate mutase OR enolase}

191

0.28

0.62

0.41

Strategy 3: transhydrogenase + {GAP DH OR phosphoglycerate kinase}

191

0.35

0.62

0.32

Wild type on glycerol

26,160

-

0.78

0.99

Strategy 1: transhydrogenase + pyruvate kinase + pyruvate DH complex + ribulose-phosphate 3-epimerase + acetate formation + glyoxylate shunt + {succinate DH OR fumarate hydratase}

22

0.38

0.68

0.40

  1. DH = dehydrogenase; ex = excretion.