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Table 3 cMCSs for E. coli: number of remaining elementary modes (EMs), minimum and maximum IPP yield as well as maximum biomass yield on glucose or glycerol [C-mol/C-mol] for different knockout strategies

From: In silico profiling of Escherichia coli and Saccharomyces cerevisiae as terpenoid factories

cMCS knockout strategy EMs Min. IPP yield Max. IPP yield Max. biomass yield
Wild type on glucose 36,590 - 0.67 0.85
Strategy 1: O2 uptake + {lactate ex OR lactate formation} + {ethanol ex OR ethanol formation OR acetaldehyde formation} + {succinate ex OR succinate OR malate DH OR fumarate hydratase} 60-86 0.38 0.48 0.08
Strategy 2: transhydrogenase + {phosphoglycerate mutase OR enolase} 191 0.28 0.62 0.41
Strategy 3: transhydrogenase + {GAP DH OR phosphoglycerate kinase} 191 0.35 0.62 0.32
Wild type on glycerol 26,160 - 0.78 0.99
Strategy 1: transhydrogenase + pyruvate kinase + pyruvate DH complex + ribulose-phosphate 3-epimerase + acetate formation + glyoxylate shunt + {succinate DH OR fumarate hydratase} 22 0.38 0.68 0.40
  1. DH = dehydrogenase; ex = excretion.