From: In silico profiling of Escherichia coli and Saccharomyces cerevisiae as terpenoid factories
cMCS knockout strategy | EMs | Min. IPP yield | Max. IPP yield | Max. biomass yield |
---|---|---|---|---|
Wild type on glucose | 36,590 | - | 0.67 | 0.85 |
Strategy 1: O2 uptake + {lactate ex OR lactate formation} + {ethanol ex OR ethanol formation OR acetaldehyde formation} + {succinate ex OR succinate OR malate DH OR fumarate hydratase} | 60-86 | 0.38 | 0.48 | 0.08 |
Strategy 2: transhydrogenase + {phosphoglycerate mutase OR enolase} | 191 | 0.28 | 0.62 | 0.41 |
Strategy 3: transhydrogenase + {GAP DH OR phosphoglycerate kinase} | 191 | 0.35 | 0.62 | 0.32 |
Wild type on glycerol | 26,160 | - | 0.78 | 0.99 |
Strategy 1: transhydrogenase + pyruvate kinase + pyruvate DH complex + ribulose-phosphate 3-epimerase + acetate formation + glyoxylate shunt + {succinate DH OR fumarate hydratase} | 22 | 0.38 | 0.68 | 0.40 |