Table 4 Primers used in this work
From: Glycolic acid production in the engineered yeasts Saccharomyces cerevisiae and Kluyveromyces lactis
Number | Sequence 5′ → 3′ |
|---|---|
O8635 | TTCTACACTGGCTACCGATTTAACTCATCTTCTTGAAAGT CTGCAGGTCGACAACCCTTA |
O8636 | TCATGATAAGATGATTCATTGCTAACTACGAAACGAAGCA CACTAGTGGATCTGATATCACCT |
O8637 | GAGTTCAATTTGTCATACCTTATCAGGCCTTATCAGCTCA CTGCAGGTCGACAACCCTTA |
O8638 | TGCAGTTGATATCACTTAGAGTATGTGTCATAGGCACGGT CACTAGTGGATCTGATATCACCT |
O8639 | TGCAGTGTCAGCCTTACGA |
O8640 | CGTTGCTATACTTTCTCGAC |
O8641 | CAAGGACACCGTCATTGAC |
O8642 | CATAGGTTTACGATTATCGAC |
O8649 | CTTTTCTTGCTTAAATCTATAACTACAAAAAACACATACA ATGCCTATCCCCGTTG |
O8650 | CATTGTTCCTTATTCAGTTAGCTAGCTGAGCTCGACTCTAG CTATTTCTTTACGCCATTTTC |
O8651 | CTATTTTCCCTTCTTACG |
O8652 | CGTTCATTGTTCCTTATTC |
O8653 | CCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGT GAATTGGGGATCTACGTATG |
O8654 | TACATTATACGAAGTTATATTAAGGGTTGTCGACCTGCAG CGAATTGGAGCTAGACAAAG |
O8655 | ATACATCCCTTTTTTTTTTTGTCTTTGTCTAGCTCCAATTCG CTGCAGGTCGACAACCCTTA |
O8656 | ACCCTCACTAAAGGGAACAAAAGCTGGAGCTCCACCGCG CACTAGTGGATCTGATATCACCT |
O8657 | CTAAATCAATCTTTTTCAATTT |
O8658 | CATACATTATACGAAGTTATATTAAGG |
O8659 | CCAGACTAATGATCAAGTAACTGTGGATTCTGCCACCGCGA GAATTGGGGATCTACGTATG |
O8660 | CTTTTTTCAATCTAGATTCCACCGCGTCAATGAACTCCTCGG CACTAGTGGATCTGATATCACCT |
O8661 | ATGTCAACAAATCTAGCAAATTACTTCGCCGGTAAGAAAG CTGCAGGTCGACAACCCTTA |
O8662 | CTAACTGCTGTCATTTCCATTTTCTTGTGGCTTGACGTCA CACTAGTGGATCTGATATCACCT |
O8643 | GTCGACGATCTACGTATGGTCATTTCTTC |
O8644 | GTCGACCGAATTGGAGCTAGACAAAG |
O8768 | CAATGAGGCTTTCTTAAGTTATGCAAGCTGTGTGTAGAGTCGTCATCCCT GCAGGTCGACAACCCTTAAT |
O8769 | GGATAAAAGCTCTATACAGACTACTATCAGAAAACTTTATTAAAGATTCA GGCCACTAGTGGATCTGATA |
O8772 | GATACTCAACGCAGTTAAGCATCTTTAGCAATCAAATTTAGCAGCCCATT GCAGGTCGACAACCCTTAAT |
O8773 | GGATATATAATGTAGCAGTAAATCGGATAGGGAAAGGCTAGCTTTCTTCT GGCCACTAGTGGATCTGATA |