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Table 3 Glycolic acid production strains constructed in this work

From: Glycolic acid production in the engineered yeasts Saccharomyces cerevisiae and Kluyveromyces lactis

Strain number

Modifications

Auxotrophies

H3744

S. cerevisiae H3675 (pGLYR1)

his3, leu2, trp1

H4241

S. cerevisiae H3675 (reg1-Δ1)

his3, leu2, trp1, ura3

H4242

S. cerevisiae H3675 (idp2Δ::ICL1)

his3, leu2, trp1, ura3

H3788

S. cerevisiae H3675 (pGLYR1, mls1-Δ1)

his3, leu2, trp1

H3790

S. cerevisiae H3675 (pGLYR1, mls1-Δ1, dal7-Δ1)

his3, leu2, trp1

H3846

S. cerevisiae H3675 (pGLYR1, mls1-Δ1, dal7-Δ1, idp2-Δ1::ICL1)

his3, leu2, trp1

H3994

S. cerevisiae H3675 (pGLYR1, mls1- Δ1, dal7-Δ1, idp2-Δ1::ICL1, reg1-Δ1)

his3, leu2, trp1

H3968

K. lactis H3954 (mls1-Δ1)

ura3 leu2 his3

H3976

K. lactis H3954 (mls1-Δ1, idp2-Δ1)

ura3 leu2 his3

H3986

K. lactis H3954 (pGLYR1, mls1-Δ1, idp2-Δ1)

leu2 his3

  1. The xylose utilising Saccharomyces cerevisiae strain H3675 (MATα his3 leu2 trp1 ura3::XYL1-XYL2 xks1::XKS1 MAL2-8c SUC2), a modified VTT C-10880, [CEN.PK113-1A [50]], was used as a parent strain for all S. cerevisiae glycolic acid production strains. The Kluyveromyces lactis strain H3954 [KHO69-8C (MATα ura3 leu2 his3::loxP ku80::loxP)] [51] was used as a parent strain for all K. lactis glycolic acid production strains.
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Microbial Cell Factories

ISSN: 1475-2859

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