Glycolic acid production in the engineered yeasts Saccharomyces cerevisiae and Kluyveromyces lactis

Table 1 Final titers of glycolic acid and biomasses in Saccharomyces cerevisiae flask cultivations

	Final titers of glycolic acid (g l^-1)			Final biomass (g_CDWl^-1)
Strain	d -glucose	d -xylose + EtOH	EtOH	d -glucose	d -xylose + EtOH	EtOH
H3744	0.03 ± 0.02	0.1 ± 0.10	0.27 ± 0.02	5.62 ± 0.04	8.08 ± 0.06	5.27 ± 0.23
H3788	0.12 ± 0.00	0.51 ± 0.02	0.59 ± 0.00	3.33 ± 0.06	4.65 ± 0.15	1.10 ± 0.03
H3790	0.11 ± 0.01	0.55 ± 0.01	0.57 ± 0.02	3.65 ± 0.06	4.43 ± 0.12	0.91 ± 0.06
H3846	0.11 ± 0.02	0.90 ± 0.01	0.60 ± 0.01	3.70 ± 0.15	4.98 ± 0.04	1.48 ± 0.07
H3994	0.10 ± 0.01	0.91 ± 0.02	0.58 ± 0.02	3.78 ± 0.06	5.35 ± 0.23	1.07 ± 0.09

Final titers and final biomasses of different glycolic acid producing S. cerevisiae strains grown in SC media containing either d-glucose (20 g l^-1), d-xylose (20 g l^-1) and ethanol (20 g l^-1), or only ethanol (20 g l^-1) as carbon sources. The d-xylose utilising Saccharomyces cerevisiae strain H3675 (MATα his3 leu2 trp1 ura3::XYL1-XYL2 xks1::XKS1 MAL2-8c SUC2), a modified [CEN.PK113-1A [50]], was used as a parent strain for all S. cerevisiae glycolic acid production strains. ± presents the standard error of the mean (SEM; n = 3).

ISSN: 1475-2859