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Table 2 Oligonucleotides used for PCR amplification in this study

From: Surface display of recombinant proteins on Escherichia coli by BclA exosporium of Bacillus anthracis

No.

Primer sequences (5′ → 3′)a

Gene to be amplified

Source or references

P1b

GGAATTC ATGCACCACCACCACCACCAC GCATTTGACCCTAATCTT

Full bclA gene

B. anthracis RA3

P2

AGTCTAGACTCGAGGCTAGCCCCGGGGGTAGGAAGGGTAAATGG

P3

CCACCATTTACCCTTCCTACCGGGCCATCCGGACTAGGACTT

Truncated bclA gene (NTD)

B. anthracis RA3

P4

AAGTCCTAGTCCGGATGGCCCGGTAGGAAGGGTAAATGGTGG

Truncated bclA gene (NTD + CTD)

B. anthracis RA3

P5

CGTCTAGACTCGAGGC TAGCCCCGG GAGCAACTTTTTCAATAA

P6

CAGGCTAGC GCTGGCACAGATTACTGGC

Bacillus sp. Endoxylanase (XynA)

[18]

P7

CCAAGCTT ATTTGTCATCGTCATCTTTATAATCCCACACTGTTACATTAGAACTTC

P8

AGAATTC ATGAAATCCCTATTGCCTACGGCAGCCGCTGGATTGTTATTACTCGCGGCCCA

P9

ATTGTTATTACTCGCGGCCCAGCCGGCCATGGCGGCTGGCACAGATTACTGGC

P10

AGAATTC ATGAAATCCCTATTGCCTACGGCAGCCGCTGGATTGTTATTACTCGCGGCCCAGCCGGCCATGGCGGCATTTGACCCTAATCTTGTAGG

P11

TGCTAG CCCCGGGGGT

P12

GGGCTAGC ATGACAATTAAAGAAATGCCTCAGCC

Bacillus megaterium Monooxygenase (P450 BM3m2)

[19]

P13

GCCCATG GCTATTATTTGTCATCGTCATCTTTATAATCCCCAGCCCACACGTC

P14

GGGAATTCA TGACAATTAAAGAAATGCCTCAGCC

  1. a Restriction enzyme sites are shown in bold.
  2. b The sequence for six histidines is shown in italic.