From: Surface display of recombinant proteins on Escherichia coli by BclA exosporium of Bacillus anthracis
No. | Primer sequences (5′ → 3′)a | Gene to be amplified | Source or references |
---|---|---|---|
P1b | GGAATTC ATGCACCACCACCACCACCAC GCATTTGACCCTAATCTT | Full bclA gene | B. anthracis RA3 |
P2 | AGTCTAGACTCGAGGCTAGCCCCGGGGGTAGGAAGGGTAAATGG | ||
P3 | CCACCATTTACCCTTCCTACCGGGCCATCCGGACTAGGACTT | Truncated bclA gene (NTD) | B. anthracis RA3 |
P4 | AAGTCCTAGTCCGGATGGCCCGGTAGGAAGGGTAAATGGTGG | Truncated bclA gene (NTD + CTD) | B. anthracis RA3 |
P5 | CGTCTAGACTCGAGGC TAGCCCCGG GAGCAACTTTTTCAATAA | ||
P6 | CAGGCTAGC GCTGGCACAGATTACTGGC | Bacillus sp. Endoxylanase (XynA) | [18] |
P7 | CCAAGCTT ATTTGTCATCGTCATCTTTATAATCCCACACTGTTACATTAGAACTTC | ||
P8 | AGAATTC ATGAAATCCCTATTGCCTACGGCAGCCGCTGGATTGTTATTACTCGCGGCCCA | ||
P9 | ATTGTTATTACTCGCGGCCCAGCCGGCCATGGCGGCTGGCACAGATTACTGGC | ||
P10 | AGAATTC ATGAAATCCCTATTGCCTACGGCAGCCGCTGGATTGTTATTACTCGCGGCCCAGCCGGCCATGGCGGCATTTGACCCTAATCTTGTAGG | ||
P11 | TGCTAG CCCCGGGGGT | ||
P12 | GGGCTAGC ATGACAATTAAAGAAATGCCTCAGCC | Bacillus megaterium Monooxygenase (P450 BM3m2) | [19] |
P13 | GCCCATG GCTATTATTTGTCATCGTCATCTTTATAATCCCCAGCCCACACGTC | ||
P14 | GGGAATTCA TGACAATTAAAGAAATGCCTCAGCC |