Table 1 Bacterial strains and plasmids used in this study
From: Surface display of recombinant proteins on Escherichia coli by BclA exosporium of Bacillus anthracis
Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
Strains | Â | Â |
E. coli JM109 | F’traD36 proA + B + lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV44 e14- gyrA96 recA1 relA1 endA1 thi hsdR17 | New England Biolabsa |
E. coli XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F’ proAB lacI qZ ΔM15 Tn10 (Tetr)]. | Stratageneb |
Plasmids | Â | Â |
pTrc99A | 4.17Â kb, bla, trc promoter | Pharmaciac |
pTac99A | 5.68Â kb, pTrc99A derivative; tac promoter | [24] |
pTJ1-BAN | 5.76Â kb, pTac99A derivative; N-terminal of bclA | This study |
pTJ1-BANC | 6.23Â kb, pTac99A derivative; N- & C-terminal of bclA | This study |
pTJ1-BAF | 6.45Â kb, pTac99A derivative; bclA | This study |
pKJX4 | pUC19 containing endoxylanase gene (xynA) | [18] |
pTJ1-BAN-XynA | 6.8Â kb, pTJ1-BAN derivative; BAN-fused xynA with His6 tag (N-terminus) and FLAG tag (C-terminus) | This study |
pTJ1-pelB-BAN-XynA | 6.36Â kb, pTJ1-BAN derivative; pelB signal peptide, BAN-fused xynA with FLAG tag (C-terminus) | This study |
pTJ1-pelB-XynA | 6.28Â kb, pTJ1 derivative; pelB signal peptide, xynA with FLAG tag (C-terminus) | This study |
pTJ1-BAN-BM3 | 9.0Â kb, pTJ1-BAN derivative; BAN-fused B. megaterium monooxygenase (P450-BM3m2) gene with His6 tag (N-terminus) and FLAG tag (C-terminus) | This study |
pTJ1-BM3 | 8.9Â kb, pTJ1-BAN derivative; B. megaterium monooxygenase (P450-BM3m2) with FLAG tag (C-terminus) | This study |