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Figure 5 | Microbial Cell Factories

Figure 5

From: Surface display of recombinant proteins on Escherichia coli by BclA exosporium of Bacillus anthracis

Figure 5

Display of BAN-fused monooxygenase (P450 BM3m2) on the cell surface. (a) SDS-PAGE analysis of E. coli displaying BAN-fused P450. Lane M, molecular weight size markers; Lanes 1 and 2, outer membrane proteins fraction of E. coli harboring pTJ1- BM3 or pTJ1-BAN-BM3, respectively. Closed and open arrowheads indicate the bands of OmpA and OmpC proteins in outer membrane proteins fraction, respectively. Arrow indicates BAN-BM3m2 (~120 kDa). (b) Confocal immunofluorescence microscope images. (i) E. coli harboring pTJ1-BAN-BM3 and (ii) E. coli harboring pTJ1-BM3. Cells were stained with FITC conjugated anti-FLAG-antibody. Each scale bar represents 3 μm.

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