Figure 1
From: Surface display of recombinant proteins on Escherichia coli by BclA exosporium of Bacillus anthracis
Cell surface display system using the BclA anchoring motif. (a) Schematic illustration of BclA on the bacterial surface. NTD, N-terminal domain of BclA; CLR, GXX triplet repeated collagen-like region; CTD, C-terminal domain of BclA. (b) The structure of the constructed surface anchoring vectors (pTJ1-BAN, pTJ1-BANC and pTJ1-BAF) with the domains of the BclA protein (NTD, gray box; CLR, black box, CTD, hatched box) and target protein (white box). Ptac; tac promoter, POI; protein of interest.