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Table 3 Results of batch fermentation with an initial glucose concentration of 90 g L -1

From: Continuous and batch cultures of Escherichia coli KJ134 for succinic acid fermentation: metabolic flux distributions and production characteristics

Time

Glucose

SA

AcA

Form A

DCW

Glucose availablea

YSPb

SA/AcA

∆SA/∆AcA

(h)

(g L-1)

(g L-1)

(g L-1)

(g L-1)

(g L-1)

(g L-1)

(g g-1)

(mol mol-1)

(g g-1)

0.0

90.0

0.0

0.0

0.0

0.07

90

-

-

-

37.5

88.0

1.9

0.4

0.1

NDc

90

0.89

2.41

2.4

47.5

87.7

2.7

0.6

0.0

0.35

91

0.79

2.29

2.0

51.5

86.2

3.7

0.8

0.0

0.80

91

0.73

2.32

2.4

67.3

75.0

11.3

2.1

0.2

1.70

89

0.79

2.77

3.0

72.0

64.0

18.7

3.2

0.2

2.18

87

0.83

2.96

3.3

80.0

49.8

28.1

4.5

0.3

2.19

82

0.87

3.18

3.7

90.0

37.3

38.5

6.1

0.5

2.05

81

0.88

3.22

3.3

109.5

20.8

49.4

7.5

0.3

NDc

NDc

NDc

3.35

3.9

119.5

16.7

53.9

7.9

0.2

1.63

75

0.93

3.45

5.1

134.5

13.9

56.2

8.2

0.2

1.45

74

0.94

3.48

4.5

145.0

12.9

57.3

8.3

0.2

1.47

74

0.94

3.50

4.5

160.5

11.8

57.6

8.3

0.2

1.39

73

0.94

3.52

 

214.5

9.8

61.0

8.7

0.2

1.64

75

0.94

3.55

4.6

  1. a Glucose present and glucose converted to product (from mass balance).
  2. b Based on calculated glucose available.
  3. c Not determined.